Gradient Program
| Time(min) | A(%) | B(%) |
| 0 | 100 | 0 |
| 18 | 90 | 10 |
| 25 | 70 | 30 |
| 39 | 70 | 30 |
| 40 | 100 | 0 |
| 50 | 100 | 0 |
| Column | Welchrom® C18, 4.6×150mm, 5μm (P/N: 00310-02041) |
| Mobile Phase | Mobile Phase A: Ammonium acetate sodium perchlorate solution( take Ammonium acetate 4.0 g and sodium perchlorate 7.0 g, dissolve in 1300 mL water, adjust pH 2.2 with H3PO4)/acetonitrile=85:15 |
| Detection | 294 nm |
| Temperature | 40℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 2 µL |
If you have any problem or require further information, please contact info@welchmat.com.
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