| Column | Ultisil® LP-C18, 5μm, 4.6×250mm PN: 00208-31043 |
| Mobile Phase | Sodium hexane sulfonate solution (0.98g hexane sulfonate, add phosphate buffer solution (6.8g potassium dihydrogen phosphate, Dissolve in water and dilute to 1000ml. Adjust pH to 2.4 with phosphoric acid))-methanol =70:30 |
| Detection | 293 nm |
| Temperature | Room temperature 40℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 10 µl |
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