System adaptability test:

ColumnWelch Ultisil LP-C18 4.6×250mm,5μm (P/N: 00208-31043)
Mobile Phase0.01M potassium dihydrogen phosphate solution adjusted to pH 2.0 with acetonitrile:phosphoric acid (97:3)
Detector280 nm
Flow Rate1.0 ml/min
Injection Volume20µl

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