Sample solution
| Column | Ultisil XB-C18 6.5×300 mm, 3 μm (P/N: 00201-21044) |
| Detection | 280 nm |
| Temperature | 35°C |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
A: Take 50mM of sodium dihydrogen phosphate, dissolve with 1000ml water, adjust pH to 4 with phosphoric acid;
B: (Take 50mM of sodium dihydrogen phosphate, dissolve with 1000ml water, adjust pH to 4 with phosphoric acid): Methanol =85:15
| Time | A(%) | B(%) |
| 0 | 90 | 10 |
| 30 | 90 | 10 |
| 35 | 0 | 100 |
| 45 | 0 | 100 |
| 46 | 90 | 10 |
| 60 | 90 | 10 |
If you have any problem or require further information, please contact info@welchmat.com.
0 Comments