Test Sample
| Column | Xtimate® C8, 4.6×250mm, 5μm |
| Mobile Phase | Ammonium phosphate solution (pH adjusted by phosphoric acid to 7.0)/acetonitrile = 50/50 Ammonium phosphate solution: accurately weigh 1.320g ammonium phosphate, add 900 ml water to dissolve, adjust pH to 7.0 with phosphoric acid; add water to 1000 mL |
| Detection | 248 nm |
| Temperature | 25 ℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 20 µL |
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