Test Sample

ColumnXtimate® C8, 4.6×250mm, 5μm
Mobile PhaseAmmonium phosphate solution (pH adjusted by phosphoric acid to 7.0)/acetonitrile = 50/50 Ammonium phosphate solution: accurately weigh 1.320g ammonium phosphate, add 900 ml water to dissolve, adjust pH to 7.0 with phosphoric acid; add water to 1000 mL
Detection248 nm
Temperature25 ℃
Flow Rate1.0 mL/min
Injection Volume20 µL

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