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Column | Ultisil XB-NH2, 5μm, 4.6×250mm (P/N 00204-31043) |
Mobile Phase | 6.81g/L KH2PO4(adjust pH to 4.7 with NaOH):Acetonitrile =35:65 |
Detection | 205nm |
Temperature | 35℃ |
Flow Rate | 1.0ml/min |
Injection Volume | 20ul |
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