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ColumnUltisil XB-NH2, 5μm, 4.6×250mm (P/N 00204-31043)
Mobile Phase6.81g/L KH2PO4(adjust pH to 4.7 with NaOH):Acetonitrile =35:65
Detection205nm
Temperature35℃
Flow Rate1.0ml/min
Injection Volume20ul

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