Test Sample
| Column | Welch Xtimate C18 4.6x250mm,5μm (P/N: 00101-21043) |
| Mobile Phase | 0.05 mol / L potassium dihydrogen phosphate (pH adjusted to 3.0 with phosphoric acid) – acetonitrile = 70:30; |
| Detector | 220nm |
| Temperature | 30℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |
| Notes | Sensitive to injection volume and dissolved sample solvent |
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