Topsil C18
| Column | Topsil C18, 4.6×250mm, 5μm (P/N 00410-01043) Ultisil AQ-C18, 4.6×150mm, 3μm (P/N 00207-21041) |
| Detection | 210nm |
| Temperature | 40℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
Mobile Phase
| Time/min | Buffer% | Acetonitrile% |
| 0 | 95 | 5 |
| 20 | 65 | 35 |
| 25 | 95 | 5 |
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