Related substance determination

ColumnUltisil XB-C18 6.0×250mm, 5μm (P/N: 00201-31043)
Mobile Phase(8mL of 10% tetrabutylammonium hydroxide, and 2.2g of disodium hydrogen phosphate, dissolve to 780mL with water): Methanol=78:22
Detection280 nm
Temperature40°C
Flow Rate1.0 ml/min
Injection Volume20 µl

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