Reference
| Column | Welch Ultisil SiO2 4.6×250，5μm (P/N: 00200-31043) |
| Mobile Phase | 0.05mol/L phosphate buffer (weigh 6.81g potassium dihydrogen phosphate dissolved in water to 1000ml, then adjust pH to 4.7 with NaOH)-acetonitrile=35:65; |
| Detector | 205nm |
| Temperature | 30℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 10ul |
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