Sample Solution
| Column | Ultisil AQ-C18, 5μm, 4.6×250mm (P/N 00207-31043) |
| Detection | 220nm |
| Temperature | 35℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
Mobile Phase
| Time | 0.02mol/L KH2PO4(adjust pH to 4.0 with phosphoric acid) | Acetonitrile |
| 0 | 98 | 2 |
| 10 | 90 | 10 |
| 25 | 65 | 35 |
| 35 | 45 | 55 |
| 40 | 35 | 65 |
| 50 | 35 | 65 |
| 55 | 98 | 2 |
| 65 | 98 | 2 |
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