SEC (Size Exclusion Chromatography)

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SEC (Size Exclusion Chromatography) is a technique that separates molecules of interest by their molecular size.  There are 2 types of SEC separation known as Gel Filtration Chromatography (uses aqueous solvents) and Gel Permeation Chromatography (uses organic solvents).  Both types of SEC use a variety of different types of stationary phase.  Welch Materials manufactures HPLC columns for SEC packed with 3 and 5 micron bonded silica media made for use when performing analytical Gel Filtration Chromatography. When considering analysis of proteins, biological enzymes, polypeptides, and other biologicals, Welch Xtimate® SEC gives the scientist a stationary phase tool produced from ultra-high purity stable silica bonded with hydrophilic polymer and diol functional groups. Welch Xtimate® SEC columns help eliminate secondary nonspecific sample interactions for a true sizing separation based on molecular size.

Features of Xtimate SEC column

• Ultra-high purity, stable silica bonded with hydrophilic polymer and diol functional groups for efficient sizing separation without secondary interactions.

•  3 or 5 µm silica microsphere for high separation efficiency.

• Highly reproducible resin and packed columns for easy method development and transfer.

• 5 pore sizes: 120 Å, 300 Å, 500 Å, 700 Å and 1000 Å separate a variety of  molecular size (weight) ranges.

Application———-Cefoxitin Sodium

Column: Xtimate®SEC-120 7.8×300 mm, 5μm

Mobile phase: phosphate buffer: acetonitrile=95:5

Flow rate: 0.9 mL/min

Column temperature: 30℃

Injection volume: 20μL

Wavelength: 232 nm

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