Sample Solution(enlarged)
| Column | Ultisil AQ-C18, 5μm, 4.6×250mm (P/N 00207-31043) |
| Detection | 220nm |
| Temperature | 40℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
Mobile Phase
A: water(adjust pH to 3.0 with acetate acid); B: acetonitrile/methanol=3/2
| Time | A | B |
| 0 | 80 | 20 |
| 40 | 30 | 70 |
| 45 | 30 | 70 |
| 50 | 80 | 20 |
| 60 | 80 | 20 |
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