Sample Solution(enlarged)

ColumnUltisil AQ-C18, 5μm, 4.6×250mm (P/N 00207-31043)
Detection220nm
Temperature40℃
Flow Rate1.0ml/min
Injection Volume20ul

Mobile Phase

A: water(adjust pH to 3.0 with acetate acid); B: acetonitrile/methanol=3/2

TimeAB
08020
403070
453070
508020
608020

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