Blank Solution
| Column | Ultisil Polar-RP, 4.6×250mm, 5μm |
| Detection | 220nm |
| Temperature | 35℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 5ul |
Mobile Phase
A: 0.2% acetic acid Solution B: acetonitrile
| Time/min | 20mm KH2PO4(pH3.5) | Acetonitrile/methanol=3/2 |
| 0 | 78 | 22 |
| 16 | 58 | 42 |
| 25 | 30 | 70 |
| 30 | 30 | 70 |
| 35 | 78 | 22 |
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