Blank Solution

ColumnUltisil Polar-RP, 4.6×250mm, 5μm
Detection220nm
Temperature35℃
Flow Rate1.0ml/min
Injection Volume5ul

Mobile Phase

A: 0.2% acetic acid Solution   B: acetonitrile

Time/min20mm KH2PO4(pH3.5)Acetonitrile/methanol=3/2
07822
165842
253070
303070
357822

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