Blank solvent
| Column | Ultisil XB-C18 6.0×250mm, 5μm, 300A (P/N: 00201-33043) |
| Mobile Phase | Buffer(Accurately weigh 28.4006g anhydrous sodium sulfate into a 1000ml beaker, dissolve it with 800ml high-purity water, add 2.7ml phosphoric acid, and adjust pH to 2.3 with ethanolamine ): acetonitrile =74:26 |
| Detection | 214 nm |
| Temperature | 40°C |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 µl |
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