Column serial number: 60181201830
| Column | Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043 |
| Detection | 231 nm |
| Temperature | 40 ℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20 µl |
Mobile Phase
| Time | Phosphate Buffer-Acetonitrile % | Acetonitrile % |
| 0 | 100 | 0 |
| 10 | 100 | 0 |
| 15 | 55 | 45 |
| 30 | 55 | 45 |
| 31 | 100 | 0 |
| 45 | 100 | 0 |
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