System adaptability solution

ColumnWelch Ultisil LP-CN 4.6×250mm,5μm (P/N: 00247-31043)
Mobile PhaseAcetonitrile: Buffer [take 1.44 g sodium lauryl sulfate and 0.75 g tetrabutylammonium bromide, add water,dissolve and dilute to 1000ml, adjust the pH to 3.0 with a 0.05mol/L sulfuric acid solution]=22:78
Detector230 nm
Flow Rate1.0 ml/min
Injection Volume20µl
NotesNeed long balance time because of ion pair reagent in mobile phase

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