System adaptability solution
| Column | Welch Ultisil LP-CN 4.6×250mm，5μm (P/N: 00247-31043) |
| Mobile Phase | Acetonitrile: Buffer [take 1.44 g sodium lauryl sulfate and 0.75 g tetrabutylammonium bromide, add water，dissolve and dilute to 1000ml, adjust the pH to 3.0 with a 0.05mol/L sulfuric acid solution]=22:78 |
| Detector | 230 nm |
| Temperature | 35℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20µl |
| Notes | Need long balance time because of ion pair reagent in mobile phase |
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