How many nights have you been by your side of a chromatography column, watching the slow flow of product from the column. There have been many hard-working scientific research doing organic synthesis, pouring the developing agent over and over again, just to recover the precious product. How many laboratories have echoed the whine of the product’s lack of purity.
For those who study organic, “Passing a column” is almost a basic skill that no one knows and no one knows. You can understand that different substances run at different speeds in the same medium, so they run for a long time. Once there will be one, two or three, we can separate them according to the order of filtering out, and obtain our products from them.
It sounds simple enough, but those who have come before, let alone those who are new to organic synthesis, often shed tears of bitterness. It is very tiring and boring to pass the column, but almost every scientific research personnel and graduate students in the field of organic have to escape from it.
The column is a very key thing in the organic laboratory. In many cases, the significance of the whole reaction can only be explained after the column is completed and the pure product is obtained. At the same time, it is also a very time-consuming and energy-consuming work: a column generally takes several hours or even longer. For example, at most a few hundred milligrams of the product will take at least one morning to complete a column; If the separation of natural extracts is more complicated, a column will often occur in a few days.
Common ways for passing a column
Just because it takes so long doesn’t mean you can just leave the column and do something else — it has to be watched all the time. You need for a period of time point plate (TLC, thin layer chromatography) to see column something out of what is now: sometimes a front between part a and part behind a four or five drops less so now, if you missed, it might be wasted, especially those in vitro is not much, use a flask or conical flask products laboratory.
Column chromatography is an indispensable separation method in organic chemistry laboratories, but general column chromatography is very troublesome. Flash chromatography column and flash chromatography system are both fast, simple and effective, and have been used more and more in laboratories at home and abroad.
Welch Materials, as a powerful manufacturer of separation and purification in the industry, offers you a Welflash, medium and low pressure preparation chromatographic column, adding a boost to your separation and purification work.
Welflash is a carefully selected medium and low pressure preparative column
- Through optimized silica gel particle size and pore diameter, Welch’s unique bonding technology ensures good reproducibility between filler batches;
- Multiple choices: Welflash column has complete types and specifications, and can have a variety of filling sizes and fillers;
- It has good compatibility with most rapid liquid chromatography instruments;
- Provide rich technical application cases.
Welflash is a column with a variety of specifications
Welflash columns are available in the following specifications: 4G, 12g, 25g, 40g, 80g, 120g, 220g and 330g.
Welflash is a chromatograph with excellent resolution