Solid Phase Extraction(SPE) is a sample pre-treatment technology, and now is now widely used. In some industries, solid phase extraction has become the main sample pre-treatment means. The mechanism of solid phase extraction mainly has non-polar effects, polar effects, cation exchange and anion exchange. How to select a suitable solid phase extraction filler according to the above mechanism, next to everyone to share the solid phase extraction packing selection Guide.
1.Non-polar Effect
Considering factors:
Low molecular weight of the target compound (<250) or weak non-polar substance
Optional packing type:
C2, C8, C18.
Considering factors:
The target compound is high molecular weight (> 250) or strong non-polar substance.
Optional packing type:
C2, C4, C6, C8, pH.
Considering factors:
A variety of neutral target compounds have different poles.
Optional packing type:
C4, C6, C8, CH, superposition (such as C2 / C18).
2.Polar effect
Considering factors:
Target compounds are polar.
Optional packing type:
Alumina, diatomaceous earth, Si, NH2, CN, diol.
Considering factors:
The target compound is non-polar or moderate polarity.
Optional packing type:
Alumina, diatomaceous earth, Si, NH2, CN, diol
3.Cation exchange
Considering factors:
The target compound is permanently with positive charge (eg quaternary ammonium).
Optional packing type:
CBA, Si, C21.
Considering factors:
The target compound PKA range is 5 ~ 10.
Optional packing type:
SCX, PRS2, CBA, C21.
Considering factors:
1, the target compound PKA range 5 ~ 10;
2, there is alkaline and neutral target compounds;
3, the sample matrix is dirty, requiring a very clean extract.
Optional packing type:
P-SCX.
4.Anion exchange
Considering factors:
The target compound is permanently with negative charge.
Optional packing type:
NH2.
Considering factors:
Target compound PKA range 2 to 6.
Optional packing type:
SAX, NH2.
Considering factors:
- Target compound PKA range 2 ~ 6;
2. There is alkaline, acidic and neutral target compound;
3. The sample matrix is dirty, requiring a very clean extract.
Optional packing type:
P-SAX.
Note
Second extraction mode
- Since C2 has a silicon hydroxyl group, it is used here as a cation exchange agent.
2. PRS can be used to replace SCX to reduce the hydrophobic force as the second force force. PRS can elute using a buffer and liquid containing an organic solvent. This can only be used with no ion-acting interference
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