|Mobile Phase||0.05mol/L phosphoric acid/triethylamine solution: acetonitrile=82:18|
|Detection||Excitation wavelength: 280nm|
Emission wavelength: 450nm
|Flow Rate||0.8 ml/min|
|Injection Volume||20 μL|
|Note||After the phosphate buffer solution was used to extract the sample and passed|
through the column, there was interference with ciprofloxacin and the recovery
rate was low. After changing to EDTA-Mcllvaine buffer solution, the interference
disappeared and the recovery rate was normal.
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