| Column | Ultisil® XB-C18(4.6×250mm,5μm) |
| Mobile Phase | 0.05mol/L phosphoric acid/triethylamine solution: acetonitrile=82:18 |
| Detection | Excitation wavelength: 280nm Emission wavelength: 450nm |
| Temperature | 30℃ |
| Flow Rate | 0.8 ml/min |
| Injection Volume | 20 μL |
| Note | After the phosphate buffer solution was used to extract the sample and passed through the column, there was interference with ciprofloxacin and the recovery rate was low. After changing to EDTA-Mcllvaine buffer solution, the interference disappeared and the recovery rate was normal. |
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