ColumnUltisil® XB-C18(4.6×250mm,5μm)
Mobile Phase0.05mol/L phosphoric acid/triethylamine solution: acetonitrile=82:18
DetectionExcitation wavelength: 280nm
Emission wavelength: 450nm
Flow Rate0.8 ml/min
Injection Volume20 μL
NoteAfter the phosphate buffer solution was used to extract the sample and passed
through the column, there was interference with ciprofloxacin and the recovery
rate was low. After changing to EDTA-Mcllvaine buffer solution, the interference
disappeared and the recovery rate was normal.

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