Substance 1 and 2

Column(Substance 1,2): Ultisil® Cellu-D, 5μm, 4.6×250mm   P/N: 00219-31043 (Substance 3,4): Ultisil® Amy-S, 5μm, 4.6×250mm    P/N: 00220-31043
Mobile Phase(Substance 1,2): Normal Hexane: Ethyl alcohol: Diethylamine =80:20:0.1 (Substance 1,2): Normal Hexane: Ethyl alcohol: Triethylamine:TFA =70:30:0.1:0.1
Detection317 nm
TemperatureRoom temperature
Flow Rate1.0 ml/min
Injection Volume4 µl
NoteMobile Phase premixed

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