| Column | Xtimate® C18, 4.6×150mm, 5μm (P/N: 00101-21041) |
| Mobile Phase | 30mm/L tetrabutylammonium bromide (adjust pH 2.5 with phosphoric acid) /methanol =60/40 |
| Detection | 234 nm |
| Temperature | Ambient |
| Flow Rate | 0.8 mL/min |
| Injection Volume | 20 µL |
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