Reference substance of related substance
Gradient Program
| Time（min） | A% | B% |
| 0 | 19 | 81 |
| 12 | 19 | 81 |
| 60 | 36 | 64 |
| Column | Welch Ultisil Polar RP 4.6×250，5μm (P/N: 00215-31043) |
| Mobile Phase | A：Acetonitrile；B：Water |
| Detector | 203nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
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