Solution of reference substance
Gradient Program
| Time（min） | A% | B% |
| 0 | 19 | 81 |
| 35 | 19 | 81 |
| 55 | 29 | 71 |
| 70 | 29 | 81 |
| 100 | 40 | 60 |
| Column | Welch Ultisil Plus C18 4.6×250mm，5μm (P/N: 00260-31043) |
| Mobile Phase | A:Acetonitrile; B:Water |
| Detector | 203nm |
| Temperature | Room Temperature |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10µl |
| Notes | After injecting the sample, the residual impurities in the column need to be eluted with acetonitrile. |
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