The third injection

ColumnXtimate® C8, 4.6×250mm, 5μm (P/N: 00102-21043)
Mobile PhasePhosphoric buffer(take monopotassium phosphate 1.2 g, dissolved in 1000 mL water, adjust pH 6.9 with potassium hydroxide)
Detection210 nm
Temperature30 ℃
Flow Rate1.2 mL/min
Injection Volume100 µL

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