The third injection
| Column | Xtimate® C8, 4.6×250mm, 5μm (P/N: 00102-21043) |
| Mobile Phase | Phosphoric buffer(take monopotassium phosphate 1.2 g, dissolved in 1000 mL water, adjust pH 6.9 with potassium hydroxide) |
| Detection | 210 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1.2 mL/min |
| Injection Volume | 100 µL |
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