11 kinds of reference substance
Gradient Program
| Time(min) | A% | B% |
| 0 | 83 | 17 |
| 15 | 78 | 22 |
| 30 | 83 | 17 |
| Column | Welch Ultisil XB-C18 4.6×250mm，5μm (P/N: 00201-31043) |
| Mobile Phase | A: 50 Mmol / L potassium dihydrogen phosphate buffer salt(pH 7.0) B: Acetonitrile |
| Detector | 254nm |
| Temperature | 25℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |
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