Reference of Folic Acid

ColumnWelch Ultisil Polar RP 4.6×250,5μm (P/N: 00215-31043)
  Mobile PhaseMethanol-10mmol/L ammonium acetate buffer (adjust pH to 3.4 with glacial acetic acid) Gradient elution: 0-1 min, 40% methanol; 1-1.01 min, 40%-95% methanol; 1.01-20 min, 95% methanol
Detector265nm
Temperature35℃
Flow Rate1.0ml/min
Injection Volume20ul
NotesMass spectrometry

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