Reference of Folic Acid
| Column | Welch Ultisil Polar RP 4.6×250，5μm (P/N: 00215-31043) |
| Mobile Phase | Methanol-10mmol/L ammonium acetate buffer (adjust pH to 3.4 with glacial acetic acid) Gradient elution: 0-1 min, 40% methanol; 1-1.01 min, 40%-95% methanol; 1.01-20 min, 95% methanol |
| Detector | 265nm |
| Temperature | 35℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
| Notes | Mass spectrometry |
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