System suitability

ColumnXtimate® C18, 4.6×250 mm, 5 μm (P/N: 00101-21043)
Mobile PhaseTake 2.0 g of potassium dihydrogen phosphate, add 650 mL of water, and add 15mL of 0.5 mol/L Tetrabutylamine hydroxide in methanol solution, 7mL of 1mol/L phosphoric acid solution and 270mL methanol, cool down, adjust pH to 5.0 with 1mol/L phosphoric acid solution or ammonia test solution, dilute with water to 1000 mL
Detection280 nm
Flow Rate1.2 mL/min
Injection Volume10 µL

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