Midbody 1

ColumnUltisil® AQ-C18 4.6×250 mm, 5 μm      P/N: 00207-31043
Mobile Phase20mmol/L dipotassium phosphate (contain 0.1% triethylamine, adjust pH to 7.2 with phosphoric acid)
Detection280 nm
Temperature30 ℃
Flow Rate1.0 ml/min
Injection Volume5 µl (midbody 2), 10µl (midbody 1 and midbody 3)
NoteIf concentration of midbody 2 too high, will lead to poor peak shape

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