|Column||Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043|
|Mobile Phase||20mmol/L dipotassium phosphate (contain 0.1% triethylamine, adjust pH to 7.2 with phosphoric acid)|
|Flow Rate||1.0 ml/min|
|Injection Volume||5 µl (midbody 2), 10µl (midbody 1 and midbody 3)|
|Note||If concentration of midbody 2 too high, will lead to poor peak shape|
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