Midbody 1
| Column | Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043 |
| Mobile Phase | 20mmol/L dipotassium phosphate (contain 0.1% triethylamine, adjust pH to 7.2 with phosphoric acid) |
| Detection | 280 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 5 µl (midbody 2), 10µl (midbody 1 and midbody 3) |
| Note | If concentration of midbody 2 too high, will lead to poor peak shape |
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