System adaptability
| Column | Welch Ultisil XB-C8 4.6×250mm，5μm (P/N: 00202-31043) |
| Mobile Phase | 1.25% diammonium hydrogen phosphate and 0.275% sodium heptane sulfonate aqueous solution – methanol = (40:60), and adjust pH to 3.5 with phosphoric acid |
| Detector | 254nm |
| Temperature | 30℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |
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