Gradient Program

Time(min)A%B%
05446
155446
171783
201783
20.015446
ColumnWelch Ultisil Polar RP 4.6×200mm,5μm (P/N: 00215-31042)
 Mobile PhaseSolution I: Take 25% tetramethylammonium hydroxide, dilute with water to 100 ml, adjust pH to 7.2 ± 0 with 85% phosphoric acid Solution II: acetonitrile-methanol = 40:60 A:Solution I – SolutionII=87.5:12.5 B:Solution I – SolutionII=12.5:87.5
Detector365nm,305nm
Temperature40℃
Flow Rate2.0ml/min
Injection Volume20ul

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