Gradient Program
| Time（min） | A% | B% |
| 0 | 54 | 46 |
| 15 | 54 | 46 |
| 17 | 17 | 83 |
| 20 | 17 | 83 |
| 20.01 | 54 | 46 |
| Column | Welch Ultisil Polar RP 4.6×200mm，5μm (P/N: 00215-31042) |
| Mobile Phase | Solution I: Take 25% tetramethylammonium hydroxide, dilute with water to 100 ml, adjust pH to 7.2 ± 0 with 85% phosphoric acid Solution II: acetonitrile-methanol = 40:60 A:Solution I – SolutionII=87.5:12.5 B:Solution I – SolutionII=12.5:87.5 |
| Detector | 365nm,305nm |
| Temperature | 40℃ |
| Flow Rate | 2.0ml/min |
| Injection Volume | 20ul |
If you have any problem or require further information, please contact info@welchmat.com.
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