Mixed sample
| Column | Welch Ultisil PFP 4.6×250mm，5μm (P/N: 00224-31043) |
| Mobile Phase | Acetonitrile-2Mmol/L sodium lauryl sulfate-glacial acetic acid=45:55:0.1 |
| Detector | 254nm |
| Temperature | 25℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10µl |
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