Blank(mobile phase)

ColumnUltisil XB-NH2, 5μm, 4.6×250mm (P/N 00204-31043)
Mobile PhaseKH2PO4(1.702g dissolved into 500ml with water): Tetrahydrofuran=25:75
Detection215nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume20ul

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