KB
Gradient Program
| Time（min） | A% | B% |
| 0 | 60 | 40 |
| 30 | 45 | 55 |
| 35 | 30 | 70 |
| 45 | 30 | 70 |
| 47 | 60 | 40 |
| 50 | 60 | 40 |
| Column | Welch Ultisil Polar RP 4.6×250mm，5μm (P/N: 00215-31043) |
| Mobile Phase | A:0.01 mol / L ammonium acetate, adjust pH to 7.0 with ammonia water ; B：Methanol: acetonitrile = 1:1; |
| Detector | 302nm |
| Temperature | 25℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20ul |
If you have any problem or require further information, please contact info@welchmat.com.
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