Reference
| Column | Ultisil ALK C18, 5μm, 4.6×250mm (P/N 00253-31043) |
| Mobile Phase | A: phosphate buffer(3.4g/L KH2PO4, adjust pH to 3.0 with phosphoric acid)/Acetonitrile=9/1 B: phosphate buffer/acetonitrile=7/13 |
| Detection | 237nm |
| Temperature | 45℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
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