System suitability solution

Gradient Program

Time(min)A(%)B(%)
01000
151000
453565
470100
550100
561000
651000
ColumnXtimate® C18, 4.6×250mm, 5μm (P/N: 00101-21043)
Mobile PhaseMobile Phase A: Acetonitrile/0.2 mol/L K2HPO4(pH 7.0)/water=35/5/60 Mobile Phase B: Acetonitrile/0.2 mol/L K2HPO4(pH 7.0)/water=60/5/35
Detection210 nm
Temperature60℃
Flow Rate1.3 mL/min
Injection Volume100 µL
NotesColumn temperature affects retention time

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