Reference substance

ColumnWelch Ultisil Polar RP 4.6×150mm,5μm (P/N: 00215-31043)
Mobile PhaseMethanol-0.01mol/L potassium dihydrogen phosphate solution (pH adjusted to 2.5 with phosphoric acid) = 17:83
Detector210 nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume10ul

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