Sample
| Column | Topsil C18, 4.6×250mm, 5μm (P/N 00410-01043) |
| Detection | 254nm |
| Temperature | Ambient |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
Mobile Phase
| Time | 0.03% trifluoroacetic acid | Acetonitrile% |
| 0 | 96 | 4 |
| 3.5 | 96 | 4 |
| 21 | 85 | 15 |
| 24 | 70 | 30 |
| 27 | 80 | 20 |
| 28 | 96 | 4 |
| 35 | 96 | 4 |
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