Test sample (Assay)

ColumnXtimate® C8, 4.6×250mm, 5μm (P/N: 00102-21043)
Mobile PhasePhosphate buffer (0.01mol/L potassium dihydrogen phosphate solution, adjust pH 2.2 with phosphoric acid) /acetonitrile=75/25
Detection215 nm
Temperature50℃
Flow Rate1.0 mL/min
Injection Volume20 µL
NotesSensitive to injection volume and dissolving solvent

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