Folic acid
| Column | Welch Ultisil XB-C18 4.6×150mm，5μm (P/N: 00201-31041) |
| Mobile Phase | Acetonitrile – 40mmol/L dipotassium hydrogen phosphate (containing 10mmol/L cetyltrimethylammonium bromide, adjust pH to 7.5 with phosphoric acid) = 40:60 |
| Detector | 215 nm |
| Temperature | 60℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
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