Gradient Program
| Time（min） | A% | B% |
| 0 | 50 | 50 |
| 30 | 45 | 55 |
| 35 | 40 | 60 |
| 40 | 40 | 60 |
| 41 | 50 | 50 |
| 51 | 50 | 50 |
| Column | Welch Ultisil Polar RP 4.6×250mm，5μm (P/N: 00215-31043) |
| Mobile Phase | A: 10mMol/L K2PO4 adjusted to pH 7.0 with phosphoric acid B：Acetonitrile |
| Detector | 224nm |
| Temperature | 30℃ |
| Flow Rate | 0.7ml/min |
| Injection Volume | 10ul |
If you have any problem or require further information, please contact info@welchmat.com.
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