Gradient Program

Time(min)A%B%
05050
304555
354060
404060
415050
515050
ColumnWelch Ultisil Polar RP 4.6×250mm,5μm (P/N: 00215-31043)
Mobile PhaseA: 10mMol/L K2PO4 adjusted to pH 7.0 with phosphoric acid B:Acetonitrile
Detector224nm
Temperature30℃
Flow Rate0.7ml/min
Injection Volume10ul

If you have any problem or require further information, please contact info@welchmat.com.


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