Sample Solution

ColumnUltisil AQ-C18, 3μm, 4.6×150mm (P/N 00207-21041)
Mobile PhaseAcetonitrile:Buffer(6ml 10% C16H37NO, add water to 200ml, adjust pH to 6.5 with phosphoric acid):Water=20:20:60
Detection254nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume20ul

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