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Doxycycline HCl on Xtimate PS DVB(EP7.0)

Sample

Column Xtimate® Phenyl-Hexyl, 7.8×250 mm, 8 μm
Mobile Phase 50 g tert-butanol, 100mL water, 200 ml pH8.0 buffer, 10g/L tetrabutyl ammonium bisulfate solution(adjust pH to 8.0 with dilute sodium hydroxide solution) 25mL, 40g/L edta solution (adjust the pH of sodium solution to 8.0 with dilute hydroxide oxidation) 5mL, add water to 500mL
Detection 254nm
Temperature 75℃
Flow Rate 2.0 mL/min
Injection Volume 20 µL
Notes This method requires high control of column temperature