Doxycycline HCl on Xtimate PS DVB(EP7.0)

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Sample

ColumnXtimate® Phenyl-Hexyl, 7.8×250 mm, 8 μm
Mobile Phase50 g tert-butanol, 100mL water, 200 ml pH8.0 buffer, 10g/L tetrabutyl ammonium bisulfate solution(adjust pH to 8.0 with dilute sodium hydroxide solution) 25mL, 40g/L edta solution (adjust the pH of sodium solution to 8.0 with dilute hydroxide oxidation) 5mL, add water to 500mL
Detection254nm
Temperature75℃
Flow Rate2.0 mL/min
Injection Volume20 µL
NotesThis method requires high control of column temperature

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1 Comment

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