| Column | Xtimate® SEC 120 (7.8×300 mm, 3 μm), 2 in series |
| Mobile Phase | 20 mM sodium dihydrogen phosphate + 20 mM disodium hydrogen phosphate + 20 mM sodium chloride, phosphoric acid adjusted to pH=5.5 |
| Detection | 280nm |
| Temperature | Room temperature |
| Flow Rate | 0.6 ml/min |
| Injection Volume | 10 μL |
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