ColumnXtimate® SEC 120 (7.8×300 mm, 3 μm), 2 in series
Mobile Phase20 mM sodium dihydrogen phosphate + 20 mM disodium hydrogen phosphate + 20 mM sodium chloride, phosphoric acid adjusted to pH=5.5
Detection280nm
TemperatureRoom temperature
Flow Rate0.6 ml/min
Injection Volume10 μL

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