Take 1ml sample and dilute it with water: acetonitrile (7:3) ten times

ColumnWelch Ultisil XB-C18 4.6×250mm,5μm (P/N: 00201-31043)
 Mobile PhaseSodium heptane sulfonate solution (Take 0.05% sodium heptane sulfonate solution and accurately weigh 5 g sodium heptane sulfonate, dissolved in 1000 ml water, then adjust pH to 2.0 with phosphoric acid) – acetonitrile = 90:10;
Detector210 nm
Flow Rate1.0ml/min
Injection Volume10ul

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