Sample Solution

ColumnXtimate® C18, 4.6×250mm, 5μm (P/N: 00101-21043)
Detection290 nm
Temperature30℃
Flow Rate1.0 mL/min
Injection Volume10 µL

Mobile Phase

Time0.1% acetic acid (%)Methanol (%)
0-1580-6520-35
15-2565-5535-45
25-3555-5045-50
35-4550-1050-90

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