Determining related substances of nadolol using Xtimate C18.

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ColumnWelch Xtimate® C18（4.6×250m)
Mobile PhaseMethanol / 0.1mol/L Sodium dihydrogen phosphate solution = 85
Detection wavelength290 nm
Column Temperature30 ℃
Flow Rate1.0 ml/min
Injection Volume20 μL
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