Test solution
| Column | Ultisil® Alk C18 (4.6×250mm,5μm) |
| Mobile Phase | Mobile phase A: take 1.2ml of 25% tetrabutylammonium hydroxide, add 1000ml of Wahaha water to prepare 0.03% tetrabutylammonium hydroxide aqueous solution, adjust the pH to 2.0 with phosphoric acid, mix well, filter by suction, and degass by ultrasonic. Mobile phase B: take chromatographic grade acetonitrile and Degas by ultrasonic. Mobile phase A / mobile phase B = 100 / 7 |
| Detection | 218nm |
| Temperature | 40℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
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