Test solution

ColumnUltisil® Alk C18 (4.6×250mm,5μm)
Mobile PhaseMobile phase A: take 1.2ml of 25% tetrabutylammonium hydroxide,
add 1000ml of Wahaha water to prepare 0.03% tetrabutylammonium
hydroxide aqueous solution, adjust the pH to 2.0 with phosphoric
acid, mix well, filter by suction, and degass by ultrasonic.
Mobile phase B: take chromatographic grade acetonitrile and Degas
by ultrasonic.

Mobile phase A / mobile phase B = 100 / 7
Detection218nm
Temperature40℃
Flow Rate1.0ml/min
Injection Volume10ul
Note/

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