Mixed standard solution
| Column | Ultisil® Alk C18（ 4.6×250mm，5μm) |
| Detection | 240nm |
| Temperature | 25℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
Mobile phase A: take 1.36g potassium dihydrogen phosphate, add 1000ml Wahaha water, mix well, filter by suction, and degass by ultrasonic.
Mobile phase B: take chromatographic grade acetonitrile.
| Time(min） | Mobile phase A (%) | Mobile phase B (%） |
| 0 | 45 | 55 |
| 15 | 45 | 55 |
| 18 | 10 | 90 |
| 25 | 10 | 90 |
| 30 | 45 | 55 |
| 40 | 45 | 55 |
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